Bio-Service

Quantitative MS and systematic biological analysis experiment service

Quantitative MS and systematic biological analysis experiment service is a next-generation proteomics experiment service featuring the use of mass spectrometer in carrying out determination of protein identity and quantitation of protein expression. Thanks to the combination of database and mass spectrometer, protein identify can be ascertained during the process of quantitation, a method which is suited to model creatures (humans, small mice, large mice, etc.) The service also offers 100 kinds of post-translation modification and analysis, analyzing, among identified proteins, post-translation modification and the relative position of the protein of your choice, according to customers' needs. Via the service, customers can access various protein expressions and possible metabolism and mechanism, thanks to systematic biological analysis. It can help customers save a lot of time for probing and trial-and-error.

Service code No. Service title Explanation Service contents Expected time
SV01

Complete experimental service for protein mass spectrometer quantitation and systematic biological analysis

    Utilize LCMSMS for analysis and quantitation of protein in specimens and systematic biological analysis for metabolism in change of protein.
  1. extraction of protein from specimens
  2.  Protein quantitation and SDS-Page electrophoresis
  3. Trypsin protein hydrolase
  4. LCMSMS mass spectrometer identification and quantitation
  5. Gene ontology systematic biological analysis
  6. 6. Metacore systematic biological analysis
 Four to six weeks
SV02

Experimental service for protein mass spectrometer quantitation, phosphorylation, and systematic biological analysis

    Purification of phosphorylation protein with column chromatographic method and analysis, quantitation of phosphorylation protein with LCMSMS, and analysis of metabolism for change in protein with systematic biological analysis
  1. Extraction of protein from specimens
  2. Purification of phosphorylation protein with column chromatographic method
  3. Phosphorylation protein quantitation and SDS-Page electrophoresis
  4. Trypsin protein hydrolase
  5. LCMSMS mass spectrometer identification and quantitation
  6. Gene ontology systematic biological analysis
  7. Metacore systematic biological
 Six to eight weeks
SV03 Experimental service for protein mass spectrometer, post-translation modification, and systematic biological analysis    Utilize column chromatographic method available on the market for purification of specific post-translation modified protein, carry out analysis and quantitation of post-translation modified protein with LCMSMS, and analyze metabolism for change in protein with systematic biological analysis.
  1. Extraction of protein from specimens
  2. Purification of post-translation modified protein
  3. Post-translation modified protein quantitation and SDS-Page electrophoresis
  4. Trypsin protein hydrolase
  5.  LCMSMS mass spectrometer identification and quantitation
  6. Gene ontology systematic biological analysis
  7. Metacore systematic biological analysis
 Six to eight weeks

 

The company's quantitative MS experiment service employs labor-free quantification method for analysis, employing liquid-chromatogram technology to separate peptide in hydrolysis from proteinase for subjection to mass-spectrum analysis. Without the need of using expensive stable isotope as internal criteria, what is needed is only to analyze mass-spectrum data generated from massive identification of protein and compare the number of corresponding peptide from different specimens before conducting relative quantitation of corresponding proteins.

 

 

 

 

 

 

Service flow​

  1. Customer consulting: We will discuss with you on experiment and offer a quote.
  2. Delivery of specimen: Customer can notify the company to collect specimen or deliver it to the company via courier.
  3. Confirmation of specimen quality: Specimen will be first subject to quality confirmation, to assure their suitability for experiment.
  4. Execution of experiment: During the process of experiment, customers can inquire about its progress.
  5. Delivery of service report: After completion of experiment, a service report and related materials will be delivered to customers.
 

Notice for service

  • For any questions on the service, please contact the company's Life Science Service Division at (Tel.: 886-2-26270835，Email：service@visualprotein.com) for discussion on details of your needs.
 

Notices for delivery of specimen​

  • Please download the document "order for quantitative MS and systematic biological  experiment service for specimen" and fill in service needs and related information on specimen, as basis for the company to carry out the service.
  • Please download and read the document "notices for preparation and delivery of specimen" before preparing specimen for analysis, along with the service order, before notifying the company to collect them or delivering them via courier to the company at Tel: 886-2-26270835, zip code: 11492, address: Fl., 6-3, No. 21, lane 583, Ruiguang Road, Neihu, Taipei, Biotech Service Division, Energenesis Biomedical)